Riboflavin Testing

TISL are able to carry out on-site testing of sprayballs within your facility to ensure full coverage of all vessel internals. With many new and re-purposed vessels having commissioning manuals missing or incomplete, evidence of the effectiveness of the sprayballs at wetting surfaces is sometimes missing. TISL are able to provide trained personnel to carry you Riboflavin Testing with Ultraviolet (UV) Light inspection to quantify the effectiveness of your sprayballs. The riboflavin testing process goes as follows:

1. Using spray equipment, the internals of the vessel are coated with a Riboflavin solution and observed under UV light. This provides a baseline to work from.
2. The system CIP pumps are then used to spray, via the system sprayballs, for a set amount of time and flow rate/ pressure.
3. The results are then observed under UV light with photographs taken for reporting. This process can be repeated at different flow rates, temperatures and for varied times to mimic the CIP process and ascertain optimal cleaning.
4. The final report will include observed removal of Riboflavin, any areas of the vessel not contacted/ wetted during the process and the impact time and flow rate/ pressure on cleanliness. All of this process is achieved without vessel entry by personnel trained in working in GMP buildings.